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Cells were washed twice with 1 PBS, trypsinized and resuspended in 3 ml of PBS and the fluorescence was immediately read on a Becton Dickinson FACScan with Cell Quest software (BD Biosciences, San Jose, USA)

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In vitro isobologram analysis The effects of tamoxifen alone and in combination with rotenone on the proliferation of drug-sensitive (AuxB1 and MDA-MD-231) and resistant (CHO R C5 and MDA-Doxo 400 ) cells were assessed using the modified fixed-ratio isobologram analysis protocol