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Oxidative stress occurs when unstable molecules called free radicals accumulate in the body and affect normal cell function

Thus pre-treatment with L-arginine counteracted the antidepressant-like effect of imipramine, venlafaxine and bupropion (Krass et al., 2011), while inhibition of nitric oxide levels within the hippocampus can induce antidepressant like effects (Joca and Guimaracs, 2006)

Its concentration declines with age from approximately 200 ng/mL in young adults to 80 ng/mL in elderly individuals a correlation that has prompted research interest in its biological functions